Twenty years ago, in an attempt to achieve successful reproduction by Lonesome George, the Galapagos National Park (GNP) decided to put two tortoises from Isabella, in George's corral. This decision was based on advice from various herpetologists. These females were selected due to their morphology, which was similar to Pinta tortoises, given that no genetic studies of Galapagos tortoises existed at that time.
However, in recent years, with the collaboration of Yale University, detailed genetics studies of Galapagos tortoises have been completed. The results of these studies indicate that although the Isabella females were morphologically similar, they are, in fact, hybrid tortoises and as such not the most ideal tortoises to mate with Lonesome George. The studies also showed that tortoises from Española are the closest genetically to Pinta tortoises (Chelonoidis abingdoni).
Therefore, they are the most compatible and will provide greater potential for reproduction with Lonesome George, with the goal of obtaining individuals with a high percentage of Pinta DNA. The two Española females have lived in captivity since the beginning of the Española breeding program. They now share a corral with Lonesome George and GNP officials are hopeful that it will be possible to obtain hatchlings sired by Lonesome George during the next reproductive season.
The breeding season is just starting and the nesting season will begin in late June. However, it is still possible for the Española females to lay eggs fertilized by Española males, so the DNA of any offspring would have to be analyzed before knowing if Lonesome George was indeed a new father.